Hadi Tavakkoli, Amin Derakhshanfar and Samaneh Noori Gooshki
Microorganisms are often the cause of many infectious diseases in human, animals and birds. In recent years, antibiotics are used in veterinary medicine to treat different types of infections. They injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease, but the adverse effects of drugs have always been a major concern. There is scantly information available about the safety and pathological alterations of tetracycline drugs in the embryonated eggs of the game birds. The objective of this study was to investigate using of oxytetracycline injectable solution for in ovo administration in partridge embryo. Fertile partridge eggs were divided into three equal treatment groups as follows: uninjected group, phosphate buffered saline-injected group and oxytetracycline-injected group whose individuals were injected with oxytetracycline injectable solution at a dosage of 10 mg per Kg egg-weight. Embryos were re-incubated post-treatment and allowed to develop until day 21 after which; they were examined for macroscopic and microscopic lesions. Results showed that embryos were normal in all treatment groups. Microscopically, no lesions were also diagnosed in tissues. Based on macroscopic and microscopic findings, it is concluded that oxytetracycline at above-mentioned concentration is not toxic for the partridge embryo. So, oxytetracycline egg-injection can be used to eliminate pathogens and prevention of egg transmission of the disease without any adverse effect.
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